![]() (D) Focused image of particles in a cell. (B and C) The transformation matrix between the focused and defocused planes. EMCCD parameter setting, image sequence acquisition and shutter control are all performed by the IQ software or Micro-Manager. HILO imaging, which provides a deeper penetration depth compared with the TIRF imaging, is generally used to image fluorescence probes inside cells at a high signal-to-noise ratio ( Li et al., 2015 Tokunaga et al., 2008). By adjusting the incident angle of the focused laser, different excitation modes can be selected: highly inclined and laminated optical sheet (HILO) and epifluorescence ( Figure 1). ![]() All laser beams are combined by dichroic mirrors and coupled into an optical fiber, and then the output light is focused at the back focal plane of the objective. The laser intensities are controlled by the laser power supplies and attenuator. Mechanical shutters (Uniblitz LS6T2, Vincent Associates) are applied to control the on−off state of these lasers. In the excitation path, different wavelengths are used: a 405 nm laser (OBIS 405−100 mW, Coherent), a 488 nm laser (Sapphire 488−200 mW, Coherent), and a 561 nm laser (Sapphire 561−200 mW, Coherent). A lens (f = 400 mm) is inserted into the path of the beam with 70% intensity to generate diffraction rings. ![]() A beam splitter is installed to divide the emission signals into two beams with a 30:70 intensity ratio, and each beam is sent to one-half of the EMCCD ( Jiang et al., 2020 Toprak et al., 2007). In the two-focal imaging part, we use the dual channel simultaneous-imaging system (DV2, Photometrics) in the emission path. Then, the piezoelectric device uses the distance as feedback control to compensate for the axial offset of the lens, thus realizing the focus-locking function. In the focal-locking part, the incident far-infrared laser (940 nm) is imaged as two spots on the CCD after total reflection at the interface between the specimen and cover glass, in which the distance between the two spots reflects the offset of the objective lens in the axial direction. To realize 3D single-particle imaging, we have added focus locking and two focal imaging apparatuses ( Figure 1). total internal reflection fluorescence (TIRF) objective lens and an ANDOR EMCCD (Ultra DU897). The 3D single-tracking system is built on an inverted Olympus IX73 microscope, which is equipped with a 60 × 1.45 N.A. It is a simple and straightforward timing light.Chemicals, peptides, and recombinant proteinsįluoSpheres™ Carboxylate-Modified MicrospheresĮpidermal growth factor with biotin conjugate There are no special features or safety features. Otherwise, there is really nothing that makes this stand out against its competitors. So if that is what you prefer, you will need to look elsewhere. It is worth noting that this is an analog timing light, not a digital. But MSD ignition lights are not the easiest to come by, which may be the reason for the higher price tag. The higher price does not really equate to a better quality. This timing light from MSD is definitely more expensive than its competitors. So if that is what you have, this is likely the best product for you. That would be expected given that this light is specifically made to work with the MSD box. The customer service from MSD is very good and they are responsive to questions or concerns. It weighs just under 2 pounds, so it is easy to handle. Its light is bright enough to be easily visible during the daylight, and it is detachable for storage. ![]() With a simple red design, MSD boasts that it is extremely accurate and tough. This timing light from MSD is top-quality for a top price. Others report that the timing light might wear out too soon. Some users say the bulb is difficult to replace when it comes time to do that. However, DIYers and professional mechanics working on vehicles with 6-volt systems might want to look elsewhere because according to some reviews, this light does not work on those systems. The instructions that are included are in English, French, and Spanish. ![]() The housing is tough ABS plastic and the heat-resistant over-molding on the battery clips allows for long service. The display also shows two- and four-cycle RPM/advance lights, and has up and down buttons. The LED tach display is digital and reads from 0-9,999 RPMs and the advance display reads to 0.10. You can use the light on all gas-powered vehicles that have electronic, conventional, computer-controlled, DIS (distributorless ignition system), and two-cycle ignition systems. The mode indicator lights give you information about RPM, advance, centrifugal advance, and vacuum advance. This timing light has a micro-processor controlled circuitry for accuracy and longevity. ![]()
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